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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to MPIF2
Only those wells that contain Human COL1Alpha1
Only those wells that contain Human TLN
The concentration of Goat BMP4 in the samples is then determined by comparing the OD of the samples to the standard curve
Only those wells that contain Rat IgG
ADP (Adiponectin) ELISA Kit (Mini-Sample) Packaging:48 wells Standards or samples are addedThis ADP (Adiponectin) ELISA Kit (Mini Sample) uses the sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to ADP . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ADP and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated.