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Biotinylated Recombinant Human Transforming Growth Factor beta 1 is produced by our Mammalian expression system and the target gene encoding Ala279-Ser390 is expressed with a Avi tag at the N-terminus
Then a biotinylated detection antibody specific for Mouse SDF-1/CXCL12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The OD value is proportional to the concentration of Human ANGPTL4
Only those wells that contain Human suPAR
The concentration of VMA in the samples is then determined by comparing the OD of the samples to the standard curve
Human TXB2 (Thromboxane B2) ELISA Kit Packaging:48 wells Biotinylated Recombinant Human Transforming GrowthThe Human TXB2 (Thromboxane B2) ELISA kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Human TXB2. During the reaction, Human TXB2 in the sample or standard competes with a fixed amount of Human TXB2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human TXB2. Excess conjugate and unbound sample or standard are washed away, and Avidin Horseradish Peroxidase
