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Substrate solution is then added and color develops in proportion to the amount of Human AOC3
Calculate the concentration of IL-35 in the sample by plotting standard curve
PHACTR1 present in the sample is added and binds to antibodies coated on the wells
Calculate the concentration of RGMA in the sample by plotting standard curve
The concentration of Mouse MYO in the samples is then determined by comparing the OD of the samples to the standard curve
Porcine ECF/CCL11 (Eosinophil Chemotactic Factor) ELISA Kit CPP32 Substrate solution is then addedThe Porcine ECF CCL11 (Eosinophil Chemotactic Factor) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Porcine ECF CCL11. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine ECF CCL11 and Avidin Horseradish Peroxidase (HRP) conjugate are added
