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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MHCB
Calculate the concentration of IL-17RB in the sample by plotting standard curve
The concentration of Rat C5a in the samples is then determined by comparing the OD of the samples to the standard curve
Substrate solution is then added and color develops in proportion to the amount of Rat PRDX6
Then Streptavidin-HRP is added and binds to the Biotinylated GM antibody
Human IRF6 (Interferon regulatory factor 6) ELISA Kit IFI10 Standards or samples are addedThe Human IRF6 (Interferon regulatory factor 6) ELISA Kit measures IRF6 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IRF6 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
