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Then Streptavidin-HRP is added and binds to the Biotinylated EPHB1 antibody
Then a biotinylated detection antibody specific for Human ICAM-1/CD54 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat OC
And then biotinylated Sheep IL12B Antibody is added and binds to IL12B in the sample
it binds with β-ENaC bound to precoated antibody
Human CFP (Properdin) ELISA Kit AXSF Then Streptavidin-HRP is added andThe Human CFP (Properdin) ELISA Kit measures CFP in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CFP antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then,
