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Mouse ACTH in the sample or standard competes with a fixed amount of Mouse ACTH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse ACTH
The OD value is proportional to the concentration of Human CTSL
The concentration of Human ELA3B in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Human PAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Mouse IgG1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse IgG1
Mouse PACAP (Pituitary Adenylate Cyclase Activating Polypeptide) ELISA Kit Packaging:96 wells Mouse ACTH in the sampleThe Mouse PACAP (Pituitary Adenylate Cyclase Activating Polypeptide) ELISA Kit measures PACAP in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti PACAP antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound
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