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You can calculate the concentration of Human DKK1 in the samples by comparing the OD of the samples to the standard curve
Only those wells that contain Human COL4
only those wells that contain Human MCP1
Only those wells that contain Human MMP-8
Then a biotinylated detection antibody specific for Rat IL-1 Beta and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Multi-Species VA (Vitamin A) ELISA Kit Packaging:48 wells You can calculate the concentrationThe VA (Vitamin A) ELISA kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Universal VA. During the reaction, Universal VA in the sample or standard competes with a fixed amount of Universal VA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal VA. Excess conjugate and unbound sample or standard are washed away, and Avidin Horseradish Peroxidase
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