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Calculate the concentration of ES in the sample by plotting standard curve
Substrate solution is then added and color develops in proportion to the amount of Chicken Ep-CAM
Then Streptavidin-HRP is added and binds to the Biotinylated RHO antibody
Then Streptavidin-HRP is added and binds to the Biotinylated SPRR2B antibody
E2- Tag antibody can recognize C-terminal
Sheep GHRL (Ghrelin) ELISA Kit WS Calculate the concentration of ESThe Sheep GHRL (Ghrelin) ELISA Kit measures GHRL in Sheep Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with GHRL. GHRL in the sample or standard competes with a fixed amount of biotin labeled GHRL for sites on a pre coated antibody specific to GHRL. Free components are washed off. After adding HRP
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