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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IL31
And then biotinylated Human IL1RAP Antibody is added and binds to IL1RAP in the sample
The concentration of Mouse NPNT in the samples is then determined by comparing the OD of the samples to the standard curve
And then biotinylated Equine GH1 Antibody is added and binds to GH1 in the sample
You can calculate the concentration of Human BECN1 in the samples by comparing the OD of the samples to the standard curve
Human PKD1 (Polycystin-1) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human (PKD1) Polycystin 1 ELISA Kit measures Polycystin 1 in samples. The plate has been pre coated with Human PKD1 antibody. PKD1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PKD1 Antibody is added and binds to PKD1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PKD1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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