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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CYS1
SERPINB12 present in the sample is added and binds to antibodies coated on the wells
The Human (ACTC1) Actin
The OD value is proportional to the concentration of Rat ET-1
And then biotinylated Mouse FASN Antibody is added and binds to FASN in the sample
Human SCG5 (Neuroendocrine Protein 7B2) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human (SCG5) Neuroendocrine Protein 7B2 ELISA Kit measures Neuroendocrine protein 7B2 in samples. The plate has been pre coated with Human SCG5 antibody. SCG5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SCG5 Antibody is added and binds to SCG5 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SCG5 antibody. After incubation unbound Streptavidin HRP is washed away
