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a horseradish peroxidase conjugated mouse anti-Human Ephrin-A1 Antibody is added to the wells and binds to Ephrin- A1 in the sample
Then a biotinylated detection antibody specific for Mouse IP-10/CXCL10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of SDF1 in the samples is then determined by comparing the OD of the samples to the standard curve
The OD value is proportional to the concentration of Rat COL3
The Mouse MUC5AC (Mucin 5 subtype AC) ELISA kit uses the Sandwich-ELISA principle
Leptin ELISA Kit UP-1 a horseradish peroxidase conjugated mouseThe Leptin ELISA kit is a two step capture or sandwich type immunoassay to measure Leptin in serum. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for leptin is immobilized onto the microplate and another monoclonal antibody specific for a different epitope of leptin is conjugated to biotin (biotin conjugate). In the first incubation step, leptin present in the specimen samples, calibrators and
