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C6orf105 present in the sample is added and binds to antibodies coated on the wells
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TNF-alpha
The concentration of Human MIP5 in the samples is then determined by comparing the OD of the samples to the standard curve
Only those wells that contain Mouse CXCL5
Then Streptavidin-HRP is added and binds to the Biotinylated AKT1 antibody
Human CST5 (Cystatin-D) ELISA Kit Packaging:96 wells C6orf105 present in the sampleThe Human (CST5) Cystatin D ELISA Kit measures Cystatin D in samples. The plate has been pre coated with Human CST5 antibody. CST5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CST5 Antibody is added and binds to CST5 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CST5 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
