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Human BMG/β2-MG in the sample or standard competes with a fixed amount of Human BMG/β2-MG on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human BMG/β2-MG
Only those wells that contain Mouse NGF
and the yield is generally about 100-400ug
The concentration of Human MIP3b in the samples is then determined by comparing the OD of the samples to the standard curve
You can calculate the concentration of Human NE/ELA2 in the samples by comparing the OD of the samples to the standard curve
Mouse CCL19/MIP3b ELISA Kit GALIG Human BMG/β2-MG in the sampleThe Mouse CCL19 MIP3b ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to MIP3b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to MIP3b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells
