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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GS
Then Streptavidin-HRP is added and binds to the Biotinylated RARRES1 antibody
Then a biotinylated detection antibody specific for Rat IFN Beta and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
And then biotinylated Rat PTH Antibody is added and binds to PTH in the sample
The Mouse (FNDC5) Fibronectin Type 3 Domain-containing Protein 5 ELISA Kit measures Fibronectin type 3 domain-containing protein 5 in Mouse samples
Human PRG4 (Proteoglycan 4) ELISA Kit SCARE1 Standards or samples are addedThe Human (PRG4) Proteoglycan 4 ELISA Kit measures Proteoglycan 4 in samples. The plate has been pre coated with Human PRG4 antibody. PRG4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PRG4 Antibody is added and binds to PRG4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PRG4 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
