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EV quantification by flow cytometry Following SEC fraction collection, EVs were labeled with anti-human BV785-conjugated CD11b antibody (Clone: M1/70, 1:1000 dilution, BioLegend), FITC-conjugated annexin V (1:1000 dilution, BioLegend), and anti-human PE-conjugated CD9 (Clone: MM2/57, 1:500 dilution, SouthernBiotech) for 20 min at 21 C and then washed in HBSS +/+ (repeated centrifugation with 16,100 g, 20 min, 4 C)
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For my frogging setup I went for the 150 XG with an 8:5:1 gear ratio
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Flexible diet (omnivory) and digestive physiology: can switch among plant material, invertebrates, and detritus as resources change
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