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Please see below for the RNA loading buffer recipe and denaturing electrophoresis conditions: RNA Loading Buffer: Deionized formamide: 200 L 10X MOPS-EDTA-Sodium Acetate Buffer (0.4 M MOPS, pH 7.0, 0.1 M sodium acetate, 10 mM EDTA): 40 L Deionized formaldehyde: 76 L Water: 14 L Denaturing Electrophoresis Conditions: 1

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This gives you a jerky drag with an on-off hard tension to light tension to hard tension over and over, and this can cause break-offs

Here is a link to technical information on this product: POR15 Bed Liner Coating (We do not sell it, and we pass this along for informational purposes only for the do-it-yourselfer who wants to save some big bucks with a little work.) Customers report that this stuff is almost bullet-proof and adheres well to our tubes, though we have not tried it ourselves